关键词: 奶山羊, 乳房炎, 病原菌, 分离鉴定, 耐药性

Abstract: [Objective] To investigate the pathogenic bacteria and drug resistance of mastitis in dairy goats in Xinxiang County.[Method]283 milk samples of dairy goats with mastitis were isolated and cultured,biochemical tests were conducted,and 16S rRNA universal primer PCR amplification was used to amplify the pathogenic bacteria,and K-B method was used to detect and analyze nine kinds of antibiotic resistance of the pathogenic bacteria.[Result]92 strains of pathogenic bacteria were isolated and identified,of which 23 strains were Staphylococcus aureus,accounting for 25.0%,11 strains were Streptococcus agalactis,accounting for 11.96%,4 strains were Streptococcus pyogenes,accounting for 4.35%,12 strains were Streptococcus uberis,accounting for 13.04%,17 strains were Escherichia coli,accounting for 18.48%,and 25 strains were other strains,accounting for 27.17%.The pathogenic bacteria have different degrees of resistance to nine antibiotics. The resistance rates of Staphylococcus aureus to Amoxicillin,Clindamycin,Cefoperazone,and Erythromycin are 65.22%,69.56%, 69.57%,60.87%,respectively.The resistance rate of Streptococci to Kanamycin is 81.48%,and that of Escherichia coli to Clindamycin and Kanamycin are 70.59% and 76.47%.[Conclusion]Staphylococcus aureus,Streptococcus and Escherichia coli were the main pathogenic bacteria of dairy goat mastitis,and the drug resistance of the pathogenic bacteria was serious.

Key words: dairy goat, mastitis, pathogenic bacteria, isolation and identification, drug resistance